Gotaq Green Master Mix Ingredients

The master mix retains all features of dreamtaq dna polymerase.
Gotaq green master mix ingredients. The system includes a proprietary dsdna binding dye carboxy x rhodamine cxr reference dye gotaq hot start polymerase mgcl sub 2 sub dntps and a proprietary reaction buffer. Gotaq green master mix is a premixed ready to use solution containing bacterially derived taq dna polymerase dntps mgcl2 and reaction buffers at optimal concentrations for efficient amplification of. Prepare one of the following reaction mixes on ice. Gotaq green master mix is a premixed ready to use solution containing bacterially derived taq dna polymerase dntps mgcl 2 and reaction buffers at optimal concentrations for efficient amplification of dna templates by pcr.
Thermo scientific dreamtaq green pcr master mix 2x is a ready to use solution containing dreamtaq dna polymerase optimized dreamtaq green buffer mgcl 2 and dntps. Component volume final conc. A protocol for a stabilized 2x master mix that includes all components for quantitative pcr except sample dna primers and water. Gotaq green master mix also includes two dyes blue and yellow that allow monitoring of progress during electrophoresis.
Gotaq green master mix contains two dyes blue and yellow that allow monitoring of progress during electrophoresis. Vortex the master mix then spin it briefly in a microcentrifuge to collect the material at the bottom of the tube. New formulation of taq dna polymerase with enhanced performance over standard taq dna polymerase. Available in two all in one master mixes.
Thaw the gotaq green master mix at room temperature. Gotaq qpcr master mixはready to useの安定した2倍濃度の試薬であり gotaq hot start polymerase mgcl 2 dntps bryt green dye 反応バッファーからなります マスターミックスには低レベルのcarboxy x rhodamine cxr が含まれますが さらに別のチューブにてcxrが付属します. The green master mix allows direct gel analysis after amplification. For a 25µl reaction volume.
High performance taq dna polymerase nucleotides dntps buffers and master mixes provide increased reliability and consistency for routine endpoint pcr. It is capable of robust amplification of up to 6 kb from genomic dna and up to 20 kb from viral dna. Gotaq green master mix 2x 12 5µl 1x. Gotaq products offer a choice of taq polymerase formulations for basic pcr hot start pcr and long range pcr.
Both master mixes are ready to use 2x solutions that contain gotaq dna polymerase dntps mgcl 2 and reaction buffer at optimal concentrations for efficient amplification of dna templates by pcr.